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Dimethyl-acetamide as a cryoprotectant for rainbow trout spermatozoa
M A McNiven1, R K Gallant, G F Richardson
1Department of Health Management Atlantic Veterinary College University of Prince Edward Island Charlottetown, P.E.I., Canada, C1A 4P3.
Theriogenology
|November 1, 1993
Summary
Dimethyl-acetamide (DMA) is a superior cryoprotectant for rainbow trout sperm compared to dimethyl sulfoxide. DMA-based extenders show promise for preserving sperm quality and fertility in aquaculture.
Area of Science:
- Aquaculture
- Reproductive Biology
- Cryobiology
Background:
- Cryopreservation of fish sperm is crucial for genetic resource management.
- Dimethyl sulfoxide (DMSO) is a commonly used cryoprotectant but can have limitations.
- Optimizing cryoprotective agents is essential for improving sperm viability and fertility.
Purpose of the Study:
- To evaluate dimethyl-acetamide (DMA) as a cryoprotectant for rainbow trout (Oncorhynchus mykiss) semen.
- To compare the efficacy of DMA with dimethyl sulfoxide (DMSO).
- To assess the potential of a novel extender formulation containing DMA.
Main Methods:
- Sperm motility assessment.
- Fluorometric determination of dead spermatozoa.
- Fertility trials with frozen-thawed spermatozoa.
- Evaluation of an extender containing NaCl, KCl, Na(2)HPO(4), L-alpha-lecithin, and DMA.
Main Results:
- Dimethyl-acetamide demonstrated significantly better cryoprotective effects than DMSO across all tested parameters (P<0.05).
- The novel extender formulation (0.137 M NaCl, 0.011 M KCl, 0.004 M Na(2)HPO(4) 7H(2)O, 7.5 g/l L-alpha-lecithin, 10% DMA) showed promise for cryopreservation.
- Rainbow trout sperm cryopreserved with DMA exhibited high motility, low dead cell percentages, and successful fertility.
Conclusions:
- Dimethyl-acetamide is a highly effective cryoprotectant for rainbow trout spermatozoa, outperforming DMSO.
- The developed extender offers a promising solution for cryopreserving rainbow trout sperm.
- Comprehensive in vitro and fertility assessments are vital for evaluating cryopreservation extenders.
