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Related Experiment Videos

Embryo cloning in sheep: work in progress.

P Loi1, S Boyazoglu, M Gallus

  • 1Istituto Zootecnico e Caseario per la Sardegna, Olmedo, Italy.

Theriogenology
|July 1, 1997
PubMed
Summary
This summary is machine-generated.

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This study demonstrates successful nuclear transfer in Sarda breed ewes using S-phase cytoplasts and a novel method to prevent embryo loss during in vivo culture, resulting in genetically identical lambs.

Area of Science:

  • Animal cloning
  • Reproductive biotechnology
  • Sheep breeding

Background:

  • Nuclear transfer is a key technique in animal cloning.
  • Optimizing embryo handling and in vivo culture is crucial for successful cloning.
  • Previous methods faced challenges in preventing embryo loss during oviduct transfer.

Purpose of the Study:

  • To refine nuclear transfer procedures using S-phase cytoplasts in Sarda ewes.
  • To introduce and evaluate a new method for securing reconstructed embryos during in vivo oviduct culture.
  • To assess the efficiency and outcomes of this improved cloning technique.

Main Methods:

  • Nuclear transfer involved transferring blastomeres from 16-cell embryos into enucleated, preactivated cytoplasts.
  • Oocytes and embryos were collected from superovulated Sarda ewes.

Related Experiment Videos

  • Reconstructed embryos were embedded in agar, transferred to oviducts, and secured with filters to prevent loss.

    • Main Results:

    • High efficiency was achieved in embryo reconstruction (100% enucleation, 98% fusion).
    • Embryo recovery from recipients was 97.3%, with 70.6% developing to blastocyst stage.
    • A 71.4% pregnancy rate and 48% survival rate at term were achieved, producing 4 clones of identical lambs.

    Conclusions:

    • The developed nuclear transfer technique with enhanced embryo retention is effective for sheep cloning.
    • This method improves the success rate of producing genetically identical animals.
    • Cloned lambs exhibited normal development, with males showing increased birth weight.