Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differences in the transmigration of different dendritic cells.

Anja Moldenhauer1, Malcolm A S Moore, Kerstin Schmidt

  • 1Institute for Transfusion Medicine, Charité-Universitätsmedizin Berlin, Berlin, Germany. moldenhauer@charite.de

Experimental Hematology
|May 27, 2006
PubMed
Summary

Hematopoietic progenitor cell (HPC)- and monocyte-derived dendritic cells (DCs) show promising migration for therapeutic use. Leukemic DCs, however, require direct lymph node injection due to limited migratory capacity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of percutaneous mechanical circulatory support to guide ablation of ventricular arrhythmias: Rationale for a national German ablation registry.

Heart rhythm·2026
Same author

Retrospective, Observational Analysis of Real-World Safety Outcomes in Patients With Locally Advanced/Metastatic Urothelial Cancer Treated With Sacituzumab Govitecan.

Clinical genitourinary cancer·2026
Same author

Longitudinal metabolomics study of phosphate-adenine-guanosine-glucose-saline-mannitol stored red blood cells.

Transfusion·2026
Same author

Ex vivo Drug Metabolism Using Primary Human Hepatocytes for the Detection of Metabolite-Dependent Antibodies to Red Blood Cells.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie·2025
Same author

[Work-related psychological stress in the public health service during and after the COVID-19 pandemic and measures for reduction].

Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·2025
Same author

Evidence-based guideline diagnosis, treatment, prevention and aftercare of oropharyngeal and hypopharyngeal carcinoma.

German medical science : GMS e-journal·2025

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Dendritic cells (DCs) are crucial for initiating immune responses.
  • The migratory capacity of DCs influences their therapeutic efficacy.
  • Understanding DC transmigration is key for optimizing cell-based therapies.

Purpose of the Study:

  • To compare the transmigration capacities of dendritic cells (DCs) derived from different sources.
  • To evaluate the potential of various DC types for therapeutic applications based on their migratory behavior.

Main Methods:

  • Generated DCs from hematopoietic progenitor cells (HPCs), peripheral blood monocytes, and leukemic cells.
  • Assessed DC transmigration in response to chemokines like macrophage inflammatory protein (MIP)-3alpha/beta and stroma-derived factor (SDF)-1alpha.

Related Experiment Videos

  • Compared morphologic features and allostimulatory capacities across DC types.

    • Main Results:

    • HPC-derived DCs exhibited the highest migration to MIP-3alpha/beta.
    • Monocyte-derived DCs showed equal attraction to MIP-3beta and SDF-1alpha.
    • Leukemic DCs displayed minimal migration to SDF-1, indicating limited homing potential.

    Conclusions:

    • While allogeneic DCs are immunocompetent, their migratory properties differ significantly.
    • HPC- and monocyte-derived DCs are suitable for subcutaneous and intravenous administration.
    • Leukemic DCs necessitate direct injection into the lymph node for optimal therapeutic effect.