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Updated: Aug 8, 2026
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
Published on: June 12, 2021
Effects of Hydrocotyle sibthorpioides extract on transplanted tumors and immune function in mice
Farong Yu1, Fahong Yu, P M McGuire
1Gansu Institute of Political Science and Law, Lanzhou 730070, China. fyu@ufl.edu
Abstract:
This paper describes the effects of an ethanolic extract of Hydrocotyle sibthorpioides on transplanted tumors and immunologic function in mice. When the H. sibthorpioides extract was administered orally at a dose of 1.5 or 3.0 g/kg body wt./day for 10 days, the inhibition rates for murine hepatic carcinoma clone (Hep), sarcoma 180 crocker clone (S(180)), and uterine cervical carcinoma clone (U(14)) were significantly enhanced. The antitumor activity of H. sibthorpioides is comparable to that of the common antitumor agent 5-fluorouracil. Also, our results indicate that the H. sibthorpioides extract promoted the thymus and spleen indices, and humoral immunity of mice. These observations demonstrated that H. sibthorpioides exerted a potent inhibitory effect on the growth of tumors, in addition to mediating immunomodulatory effects in mice.
Insights
The ethanolic extract of Hydrocotyle sibthorpioides demonstrated significant antitumor activity against transplanted tumors in mice. This natural compound also enhanced immune function, suggesting potential as a novel cancer therapeutic.
Area of Science:
- Pharmacology
- Immunology
- Natural Product Research
Background:
- Traditional medicine often utilizes plant extracts for therapeutic purposes.
- Investigating natural compounds for cancer treatment is a growing area of research.
- Understanding the immunomodulatory effects of natural products is crucial for drug development.
Purpose of the Study:
- To evaluate the antitumor effects of Hydrocotyle sibthorpioides ethanolic extract on transplanted tumors in mice.
- To assess the impact of the extract on the immunologic function of mice.
- To compare the efficacy of Hydrocotyle sibthorpioides with a conventional chemotherapy agent.
Main Methods:
- Oral administration of Hydrocotyle sibthorpioides ethanolic extract (1.5 or 3.0 g/kg) daily for 10 days.
- Assessment of tumor inhibition rates for murine hepatic carcinoma (Hep), sarcoma 180 (S(180)), and uterine cervical carcinoma (U(14)).
- Evaluation of thymus and spleen indices and humoral immunity in treated mice.
Main Results:
- Significant enhancement in the inhibition rates of Hep, S(180), and U(14) tumors.
- Antitumor activity comparable to the established agent 5-fluorouracil.
- Promotion of thymus and spleen indices, indicating enhanced immune organ health.
- Improvement in humoral immunity parameters in mice treated with the extract.
Conclusions:
- Hydrocotyle sibthorpioides ethanolic extract exhibits potent inhibitory effects on transplanted tumor growth in mice.
- The extract demonstrates significant immunomodulatory effects, enhancing both organ indices and humoral immunity.
- These findings suggest Hydrocotyle sibthorpioides holds promise as a natural therapeutic agent for cancer treatment.
