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Related Experiment Videos

Oncoprotein immunoreactivity in human endocrine tumours.

M Roncalli1, D R Springall, I M Varndell

  • 1Department of Histochemistry, Royal Postgraduate Medical School, London, U.K.

The Journal of Pathology
|February 1, 1991
PubMed
Summary

Oncogene expression was investigated in endocrine tumors using immunocytochemistry. Specific oncoproteins were detected in various tumor types, suggesting their potential for identifying distinct tumor subsets.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Endocrinology

Background:

  • Abnormally expressed oncogenes play a role in neoplastic transformation.
  • Immunocytochemistry is a valuable tool for screening oncogene expression in tumors.

Purpose of the Study:

  • To investigate oncogene expression in a series of endocrine tumors.
  • To determine if specific oncoproteins correlate with tumor type or histopathological findings.

Main Methods:

  • Immunocytochemistry was used to detect c-erbB-2, c-myc, L-myc, and N-myc oncoproteins.
  • Paraffin sections of pulmonary small cell carcinomas, atypical carcinoids, bronchial carcinoids, medullary thyroid carcinomas, phaeochromocytomas, and insulinomas were analyzed.

Main Results:

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  • Oncoprotein expression varied across tumor types: c-erbB-2 in small cell carcinomas and phaeochromocytomas, c-myc in small cell carcinomas and atypical carcinoids, N-myc in medullary thyroid carcinomas, and L-myc in phaeochromocytomas.
  • Significant intratumoral and intertumoral heterogeneity was observed.
  • No correlation was found between tumor pattern, mitotic index, and oncoprotein immunoreactivity.

    • Conclusions:

    • Oncogene products are present in a subset of endocrine tumors.
    • Specific oncoproteins appear to be associated with particular tumor types.
    • Oncoprotein detection may aid in identifying morphologically distinct endocrine tumor subsets.