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P1 protein of Cocksfoot mottle virus is indispensable for the systemic spread of the virus

Merike Meier1, Heiti Paves, Allan Olspert

  • 1Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia. merike.meier@ttu.ee

Virus Genes
|May 30, 2006
PubMed

Insights

Cocksfoot mottle sobemovirus P1 protein is essential for systemic infection in oat plants, though not for initial replication. Its absence significantly reduces virus accumulation and movement.

Area of Science:

  • Plant Virology
  • Molecular Biology
  • RNA Viruses

Background:

  • Cocksfoot mottle sobemovirus (CfMV) is an RNA virus with a 5' ORF1 encoding a non-conserved protein, P1.
  • The specific functions of the CfMV P1 protein remain largely uncharacterized.

Purpose of the Study:

  • To investigate the role of the CfMV P1 protein in viral replication, systemic infection, and cell-to-cell movement.
  • To determine the subcellular localization of the CfMV P1 protein.

Main Methods:

  • Generation and analysis of P1-deficient CfMV mutants.
  • Infection assays in oat leaves and barley suspension cell cultures.
  • Confocal microscopy to observe CfMV P1:EGFP localization in Sf9 insect cells.

Main Results:

  • P1-deficient CfMV replicated in oat leaves and barley cells but failed to infect oat plants systemically.
  • The absence of P1 significantly reduced virus accumulation efficiency.
  • CfMV P1 demonstrated limited cell-to-cell movement and localized to membranes and inclusions in insect cells.

Conclusions:

  • The CfMV P1 protein is crucial for systemic spread and efficient accumulation in host plants.
  • P1 plays a role in viral movement, potentially mediating cell-to-cell transport.
  • Subcellular localization suggests P1 interacts with cellular membranes or viral inclusions.

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