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Published on: January 7, 2019
Evaluation of methanol-water and acetonitrile-water binary mixtures as eluents for temperature-dependent inclusion
Paweł K Zarzycki1, Elzbieta Włodarczyk, Da-Wei Lou
1Laboratory of Toxicology, Department of Environmental Biology, Technical University of Koszalin, Poland. pkzarz@wp.pl
Abstract:
In the present study the solubility of beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin at sub-zero and elevated temperatures (-10 and +30 degrees C) for a given composition of methanol/water and acetonitrile/water binary mixtures (Xs = 0.16) was studied. Moreover, the freezing temperature profiles of acetonitrile-based chromatographic mobile phases were measured, and the obtained results were compared with data available in the literature. Furthermore, the effect of the macrocycles concentration on the liquid-phase freezing points was determined. The low solubility of native beta-cyclodextrin in a methanol/water mixture at sub-zero temperature as well as the non-linear behavior of acetonitrile/water mixtures that were observed concerning the freezing point profile are discussed from a practical point of view.
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