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Evolutionary potential of a duplicated repressor-operator pair: simulating pathways using mutation data.

Frank J Poelwijk¹, Daniel J Kiviet, Sander J Tans

¹FOM Institute for Atomic and Molecular Physics (AMOLF), Amsterdam, Netherlands.

Plos Computational Biology

|May 31, 2006

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Gene duplication allows new functions to evolve. This study shows duplicated repressors can gain new DNA binding sites through co-divergence, driven by selection, not neutral mutations.

Area of Science:

Evolutionary biology
Molecular biology
Systems biology

Background:

Gene duplication is crucial for evolution, but the divergence process remains unclear.
Understanding how duplicated genes acquire new functions is essential for deciphering regulatory network evolution.

Purpose of the Study:

To computationally investigate the step-by-step divergence of duplicated repressors.
To identify selective conditions enabling the evolution of new regulatory interactions.

Main Methods:

Simulated gene duplication of repressors in Escherichia coli.
Quantified intermediate phenotypes using in vivo repression strengths of lac mutants.
Constructed evolutionary pathways via base pair substitutions and selection for binding.

Related Experiment Videos

Main Results:

Duplicated repressors and their binding sites co-evolve, facilitating new interactions.
Early fitness increases observed during divergence towards new regulatory roles.
Neutral mutations were found to be non-essential for significant divergence.

Conclusions:

Coevolutionary mechanisms are vital for the divergence of regulatory networks.
The study highlights key factors driving the evolution of protein-DNA and protein-protein interactions post-duplication.