Related Experiment Video
Updated: Aug 8, 2026
Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids
Published on: March 10, 2023
[Cryopreserved human heart cell transplantation--effect of cryopreservation on cell proliferation and immunogenicity]
Hiroki Yokomuro1, Noritsugu Shiono, Tsukasa Ozawa
1Division of Cardiovascular Surgery, Department of Surgery School of Medicine, Faculty of Medicine, Toho University.
Unlabelled:
We studied the possibility that cryopreserved human heart cells could be transplanted with some advantage.
Method:
Cells derived human atrial tissues (control group (n = 13)), cryopreserved cells (cell-cryopreservation group (n = 23), and cells derived cryopreserved tissue (tissue-cryopreservation group (n = 29)) were cultured for 15 days. The cell proliferation was compared between control and cryopreservation group by growth curves. BasicFGF, TGFbeta-1, IL-6, IL-8, and cell cycle were measured at pre and post-cryopreservation. Mixed Lymphocyte-heart cell culture established by PBL and heart cells was evaluated by PBL proliferation. Transplanted cells were evaluated by visually and histology.
Results:
Cryopreserved cells were proliferated much more than control cells (p < 0.0001). The growth factors were increased, and cytokines were decreased by cryopreservation (p < 0.05). The cryopreserved cell cycles were shifted to G2 + M from G1 + G0 period. Cryopreserved cells stimulated PBL less than non-cryopreserved cells (p < 0.0001). Transplanted cryopreserved cell were survived.
Conclusion:
Cryopreserved human heart cells can be transplanted, and proliferated much more than non-cryopreserved cells. Cryopreservation enables the human cells to be more prolific, and reduced the immunogenicity of them. The transplanted cryopreserved cells survived and formed cardiac-like tissue.
