Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enhanced transdermal-immunization with diptheria-toxoid using local hyperthermia.

Pramod Upadhyay1

  • 1National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India. pkumar@nii.res.in

Vaccine
|June 1, 2006
PubMed
Summary

Mild local hyperthermia enhances transdermal immunization by increasing antigen transport and immune cell activation. This novel method generates antibody responses comparable to traditional injections, offering a painless alternative.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Monocyte Transcriptome in Different Phases of Chronic Hepatitis B Virus Infection Uncovers Potential Functional Roles.

Viral immunology·2024
Same author

Reconditioned monocytes are immunomodulatory and regulate inflammatory environment in sepsis.

Scientific reports·2023
Same author

Microdissection of the Rodent Eye.

Journal of visualized experiments : JoVE·2023
Same author

Immunological consequences of compromised ocular immune privilege accelerate retinal degeneration in retinitis pigmentosa.

Orphanet journal of rare diseases·2022
Same author

The safety and efficacy of BCG encapsulated alginate particle (BEAP) against M.tb H37Rv infection in Macaca mulatta : A pilot study.

Scientific reports·2021
Same author

Mean core to peripheral temperature difference and mean lactate levels in first 6 hours of hospitalisation as two indicators of prognosis: an observational cohort study.

BMC pediatrics·2020

Area of Science:

  • Immunology
  • Dermatology
  • Biotechnology

Background:

  • Mild local hyperthermia activates the immune system.
  • Hyperthermia facilitates the transdermal transport of high molecular weight molecules and nanoparticles.
  • It upregulates co-stimulatory molecules like CD80 and CD86, crucial for immune responses.

Purpose of the Study:

  • To investigate the efficacy of mild local hyperthermia for transdermal immunization.
  • To assess the immune response generated by hyperthermia-enhanced transdermal antigen delivery.
  • To compare this novel immunization route with conventional methods.

Main Methods:

  • Mice were immunized transdermally with diphtheria toxoid under local hyperthermia.
  • No adjuvants or penetration enhancers were used.

Related Experiment Videos

  • Immune response was evaluated via antibody production and splenocyte proliferation assays.

    • Main Results:

    • Transdermal immunization with diphtheria toxoid under local hyperthermia induced a significant antibody response.
    • Splenocytes from immunized mice showed proliferation upon re-exposure to the antigen, indicating immunological memory.
    • The immune response was comparable to that achieved via conventional intramuscular injection.

    Conclusions:

    • Mild local hyperthermia is a viable strategy for effective transdermal immunization.
    • This method bypasses the need for adjuvants and painful injections.
    • Hyperthermia-enhanced transdermal immunization presents a promising, patient-friendly alternative for vaccine delivery.