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Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
Published on: August 27, 2021
Molecular addresses of tumors: selection by in vivo phage display
Xiao-Bo Li1, Hermann J Schluesener, Shun-Qing Xu
1MOE Key Lab of Environment and Health, Institute of Environmental Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. China.
Abstract:
In vivo phage display has been used extensively to screen for novel targets of tumor therapy. Phage display peptide libraries can express random peptides or protein fragments and the aim of phage display is to identify peptide molecules that bind stably to a given target. Angiogenesis is essential to tumor development. Both blood and lymphatic vessels of tumors are different from those of normal tissues. Phage display has been used to analyze the structure and molecular diversity of tumor vasculature and to select tumor-specific antigens which have revealed stage- and type-specific markers of tumor blood vessels. Furthermore, peptides identified by in vivo phage display also work as vehicles to transport cargo therapeutic reagents to tumors. These peptides and their corresponding cellular proteins and ligands may provide molecular tools to selectively target the addresses of tumors and their pathological blood vessels and might increase the efficacy of therapy while decreasing side effects.
Insights
In vivo phage display identifies tumor-specific peptides for targeted cancer therapy. These peptides can deliver therapeutic agents to tumors, enhancing treatment efficacy and reducing side effects.
Area of Science:
- Biotechnology
- Oncology
- Molecular Biology
Background:
- In vivo phage display screens for novel tumor therapy targets.
- Angiogenesis is crucial for tumor development, with distinct tumor vasculature.
- Phage display analyzes tumor vasculature and identifies tumor-specific antigens.
Purpose of the Study:
- To identify peptide molecules that bind stably to tumor targets.
- To analyze the structure and molecular diversity of tumor vasculature.
- To select tumor-specific antigens for targeted therapy.
Main Methods:
- Utilizing in vivo phage display with peptide libraries.
- Screening for peptides that bind to tumor vasculature.
- Identifying stage- and type-specific markers of tumor blood vessels.
Main Results:
- Identified peptides that bind specifically to tumor vasculature.
- Discovered stage- and type-specific markers of tumor blood vessels.
- Peptides can function as vehicles for delivering therapeutic reagents to tumors.
Conclusions:
- In vivo phage display is effective for identifying tumor-specific antigens.
- Selected peptides can serve as molecular tools for targeted tumor therapy.
- Targeted delivery may increase therapeutic efficacy and decrease side effects.
