Related Experiment Video
Updated: Aug 8, 2026

Scalable, Flexible, and Cost-Effective Seedling Grafting
Published on: January 6, 2023
Gibberellins and antheridiogen on sex in Blechnum spicant L
V Menéndez1, M A Revilla, P Bernard
1Laboratory of Plant Physiology, Department of BOS, c) Catedrático R. Uría s/n, 33071 Oviedo, Spain.
Abstract:
The role of gibberellins (GAs) in determining sex in the gametophyte of the fern Blechnum spicant L. was studied through (a) the effect of exogenous GA(4+7) and GA3 (b) quantitation of the endogenous levels of GA1, GA3, GA4, GA7, GA9, and GA20 in male and female gametophytes, and (c) the effect of flurprimidol, a GAs biosynthesis inhibitor of the steps of oxidation of ent-kaureno to ent-kaurenoic acid. Our results show that GA(4+7) had a slight effect of inducing either male or female sexual organs, antheridia and archegonia, respectively. The endogenous GAs content was not significantly different between sexes, but the GA4, GA7, and GA20 levels were raised above those of the other GAs in both sexes. Neither antheridiogen biosynthesis nor antheridia formation was inhibited by flurprimidol. Gametophytes regenerated from homogenized mature gametophytes (HG) show a different physiological behavior than spore-derived gametophytes. In the first case, gametophytes are males and synthesize antheridiogen before they attain maturity, in contrast to what occurs in spore-derived gametophytes which are females and synthesize antheridiogen when mature.
More Related Videos
11:56Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
Published on: April 17, 2009
07:19Asymbiotic Germination and Leaf Explant-Based Regeneration of the Endangered Medicinal Orchid Hemipilia cucullata from Mature Seeds
Published on: September 19, 2025
Related Concept Videos
The Angiosperm Life Cycle
Morphogenesis
Plant Hormones
Asexual Reproduction
Cell Signaling in Plants
Yeast Signaling