Quantitative changes in branchial carbonic anhydrase activity and expression in the euryhaline green crab, Carcinus...

Hemolymph osmolality, and changes in gill carbonic anhydrase (CA) activity, relative mRNA expression, and CA protein concentration were measured in the green crab Carcinus maenas acclimated to 32 ppt salinity and transferred to 10 ppt. Hemolymph osomolality stabilized at new, acclimated values, by 24 hr after transfer. There was a large increase in CA mRNA concentrations, as measured by quantitative PCR, in the posterior gills by 24 hr post-transfer that remained elevated through 4 days. By 7 days, however, CA mRNA levels began to decline. CA activity, on the other hand, did not begin to increase until 48 hr after transfer to 10 ppt, but it continued to increase through 7 days. CA protein concentration increased by 5-fold in posterior gills in crabs acclimated to 10 ppt. CA activity, mRNA expression, and CA protein concentrations did not change in anterior gills. These results indicate that low salinity-stimulated CA induction is under transcriptional regulation, and that the increase in CA activity is a result of the increase in gene expression and synthesis of new enzyme. Changes in mRNA appear to be transient, but once synthesized, the CA protein appears to persist in the gill for an extended time. In a separate set of experiments, green crabs acclimated to 32 ppt were transferred directly to salinities of 25, 20, 15, and 10 ppt. CA activity and mRNA concentrations increased with decreasing salinity, peaking at 15 ppt but decreasing between 15 and 10 ppt. The decrease may represent a breakdown in the mechanism of transport-related protein induction near the lower salinity limit of this species.