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Related Experiment Videos

The human embryo in vitro: recent progress.

Susanne Geary1, Young S Moon

  • 1Gamete and Embryo Laboratory, University of British Columbia Centre for Reproductive Health, 805 West 12th Avenue, Vancouver, B.C., Canada V5Z 1M9. sgeary@interchange.ubc.ca

The Journal of Reproductive Medicine
|June 2, 2006
PubMed
Summary
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This review examines advanced laboratory techniques for in vitro fertilization (IVF), including embryo culture, intracytoplasmic sperm injection (ICSI), and preimplantation genetic diagnosis, to improve fertility outcomes.

Area of Science:

  • Reproductive Medicine
  • Embryology
  • Genetics

Background:

  • In vitro fertilization (IVF) and associated treatments have significantly advanced over the past two decades.
  • Continuous improvements in laboratory procedures are crucial for enhancing the success rates of assisted reproductive technologies.
  • Evaluating the clinical efficacy of these evolving techniques is essential for patient care.

Purpose of the Study:

  • To review current laboratory techniques and procedures employed in in vitro fertilization (IVF).
  • To assess the efficacy and outcomes of various associated treatment methods.
  • To highlight advancements in embryo culture, ICSI, assisted hatching, cryopreservation, PGD, and IVM.

Main Methods:

  • Comprehensive review of peer-reviewed articles focusing on IVF and related laboratory procedures.

Related Experiment Videos

  • Analysis of techniques including sequential media for blastocyst culture, intracytoplasmic sperm injection (ICSI), assisted hatching, cryopreservation, preimplantation genetic diagnosis (PGD), and in vitro maturation (IVM).
  • Evaluation of reported outcomes and clinical applications of these methods.

    • Main Results:

    • Sequential media enable embryo culture to the blastocyst stage.
    • Intracytoplasmic sperm injection (ICSI) improves outcomes in male factor infertility, producing more embryos with higher implantation rates.
    • Assisted hatching shows potential benefits for specific patient groups, though standardized criteria are lacking. Preimplantation genetic diagnosis (PGD) aids in preventing genetic disorder transmission. Successful pregnancies have been reported following in vitro maturation (IVM).

    Conclusions:

    • IVF and associated laboratory techniques have seen substantial development and improvement over the last 20 years.
    • Techniques like ICSI and advancements in embryo culture have demonstrably improved fertility treatment success.
    • Further evaluation of the clinical benefits and standardization of procedures like assisted hatching are necessary.