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Proteomics-based expression library screening (PELS): a novel method for rapidly defining microbial immunoproteomes
Indira T Kudva1, Bryan Krastins, Haiqing Sheng
1Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
Molecular & Cellular Proteomics : MCP
|June 2, 2006
Summary
This study introduces proteomics-based expression library screening to identify microbial proteins. This novel method rapidly defines microbial immunoproteomes for diagnostic and therapeutic applications.
Area of Science:
- Microbiology
- Immunology
- Proteomics
Background:
- Current methods for identifying microbial proteins that trigger immune responses are limited, especially in the post-genomic era.
- There is a need for efficient techniques to analyze microbial immunoproteomes.
Purpose of the Study:
- To introduce and validate a novel proteomics-based expression library screening method.
- To rapidly identify microbial proteins with diagnostic, prophylactic, and therapeutic potential.
Main Methods:
- Developed a proteomics-based expression library screening approach.
- Applied the method to Escherichia coli O157:H7 in bovine reservoirs.
Main Results:
- Successfully identified 207 proteins from the Escherichia coli O157:H7 immunome.
- Demonstrated proof-of-principle for the novel screening technique within 3 weeks.
Conclusions:
- Proteomics-based expression library screening is a broadly applicable and rapid method for defining microbial immunoproteomes.
- This technique is ideal for mining microbial proteomes for potential diagnostic, prophylactic, and therapeutic targets.