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Effects of steroid hormones on replication of murine sarcoma virus in mouse embryo cultures
Abstract:
The androgenic steroid hormone, testosterone, inhibited focus formation by the murine Moloney sarcoma virus in mouse embryo cells. The inhibition of focus formation was enhanced by cyclic AMP. Although focus formation was inhibited, there was no inhibition of viral replication. The glucogenic adrenal corticosteroids, cortisol and dexamethasone, and 17-beta-estradiol and progesterone did not affect focus formation by MuSV(M).
Insights
Testosterone and cyclic AMP inhibit Moloney sarcoma virus focus formation in mouse cells, but do not affect viral replication. Other steroid hormones showed no effect on this viral process.
Area of Science:
- Endocrinology
- Virology
- Cell Biology
Background:
- Steroid hormones play crucial roles in various physiological processes.
- Viral oncogenesis involves complex interactions between viruses and host cells.
- The role of specific hormones in modulating viral transformation is an area of ongoing research.
Purpose of the Study:
- To investigate the effect of testosterone on focus formation by Moloney sarcoma virus (MuSV(M)) in mouse embryo cells.
- To determine if cyclic AMP modulates testosterone's effect on viral focus formation.
- To assess the impact of other steroid hormones on MuSV(M) focus formation.
Main Methods:
- Mouse embryo cells were exposed to MuSV(M) in the presence of various steroid hormones.
- Focus formation, a measure of viral transformation, was quantified.
- Viral replication was monitored to distinguish between inhibition of transformation and replication.
Main Results:
- Testosterone significantly inhibited focus formation by MuSV(M).
- Cyclic AMP enhanced the inhibitory effect of testosterone on focus formation.
- Cortisol, dexamethasone, 17-beta-estradiol, and progesterone did not affect focus formation.
- Viral replication was not inhibited by testosterone or cyclic AMP.
Conclusions:
- Testosterone, potentially modulated by cyclic AMP, can inhibit the transforming ability of Moloney sarcoma virus.
- The observed inhibition is specific to viral transformation and not viral replication.
- Other tested steroid hormones do not influence MuSV(M) focus formation.