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Working with Human Tissues for Translational Cancer Research
07:48

Working with Human Tissues for Translational Cancer Research

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InteXen tissue processing and laboratory study

J Christian Winters1

  • 1Urodynamics and Female Urology, Ochsner Clinic Foundation, New Orleans, LA, USA. cwinters@ochsner.org

International Urogynecology Journal and Pelvic Floor Dysfunction
|June 2, 2006
PubMed

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...