Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene identification and cloning by molecular marker mapping.

Georg Jander1

  • 1Boyce Thompson Institute for Plant Research, Ithaca, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2006
PubMed
Summary

Map-based cloning in Arabidopsis identifies mutant genes using genetic mapping and DNA sequencing. This efficient process, aided by a complete genome sequence, allows for gene identification within approximately one year.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Independent recruitment of enzymes for phenylpropanoid sucrose ester biosynthesis in Polygala tenuifolia.

The Plant journal : for cell and molecular biology·2026
Same author

Basic Research on Whitefly Molecular Biology: A Foundation for Innovative Pest Management Strategies.

Current issues in molecular biology·2026
Same author

Unlocking the potential of <i>Pseudomonas aeruginosa</i> QS intermediates as antimicrobial synergists against three multidrug-resistant enteric bacteria.

Frontiers in microbiology·2026
Same author

A combination of cry (Cry2Ab) and plant lectin (PTA) genes confers resistance against major sucking and chewing insect pests of cotton.

Molecular biology reports·2025
Same author

ZmPP2C45 and ZmBELL4 suppress maize biochemical defense against insect herbivores.

The New phytologist·2025
Same author

In situ foliar augmentation of multiple species for optical phenotyping and bioengineering using soft robotics.

Science robotics·2025

Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • Map-based cloning is a key method for identifying genes responsible for specific phenotypes in model organisms.
  • The availability of a fully sequenced genome and numerous molecular markers significantly streamlines this process in Arabidopsis thaliana.

Purpose of the Study:

  • To outline the established four-stage process of map-based gene cloning in Arabidopsis.
  • To highlight the efficiency and feasibility of completing map-based cloning projects within a year.

Main Methods:

  • Induction of mutations and identification of plants with desired phenotypes.
  • Crossing mutant plants with wild-type ecotypes to generate F2 populations for genetic analysis.
  • Genotyping and phenotyping of F2 populations to establish linkage to molecular markers and narrow down the chromosomal region.
  • DNA sequencing of mutant and wild-type samples to pinpoint the causative gene.

Main Results:

  • The described four-stage process enables precise localization of mutant genes to small chromosomal regions.
  • Successful map-based cloning projects in Arabidopsis can be completed in approximately one year.

Conclusions:

  • Map-based cloning is a robust and efficient strategy for Arabidopsis functional genomics.
  • The combination of genetic mapping, molecular markers, and genome sequencing facilitates rapid gene discovery.

Related Experiment Videos