Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear pore complex structure and plasticity revealed by electron and atomic force microscopy.

Bohumil Maco1, Birthe Fahrenkrog, Ning-Ping Huang

  • 1M. E. Müller Institute for Structural Biology, Biozentrum, University of Basel, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2006
PubMed
Summary

Electron microscopy and atomic force microscopy reveal the 3D structure and dynamics of nuclear pore complexes (NPCs). These techniques offer insights into the supramolecular machine controlling nucleocytoplasmic transport.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation of M2 macrophage membrane hybrid lipid nanoparticles based on microfluidic device for atherosclerosis treatment.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Advanced technologies of artery-on-a-chip: a review of construction strategies and disease models.

Angiogenesis·2026
Same author

A Spinster-like Transporter at the Inner Membrane Complex is critical for Toxoplasma gondii cytokinesis, motility and invasion.

PLoS pathogens·2026
Same author

RNG2 tethers the conoid to the apical polar ring in Toxoplasma gondii to enable parasite motility and invasion.

PLoS biology·2025
Same author

Construction of engineered cardiac tissue on a heart-on-a-chip device enables modeling of arrhythmogenic right ventricular cardiomyopathy.

Biosensors & bioelectronics·2025
Same author

Dissecting apicoplast functions through continuous cultivation of Toxoplasma gondii devoid of the organelle.

Nature communications·2025

Area of Science:

  • Cell Biology
  • Biophysics
  • Structural Biology

Background:

  • Nuclear pore complexes (NPCs) are crucial supramolecular machines regulating nucleocytoplasmic transport.
  • Understanding NPC structure and function is vital for comprehending cellular processes.

Purpose of the Study:

  • To explore the ultrastructure and dynamics of nuclear pore complexes (NPCs).
  • To highlight the utility of various microscopy techniques in studying NPCs.

Main Methods:

  • Electron microscopy (EM) techniques including negative staining, cryoelectron microscopy, and tomography.
  • Atomic force microscopy (AFM) for investigating NPC dynamics under physiological conditions.
  • Combination of EM and AFM with immunolabeling for detailed structural analysis.

Related Experiment Videos

Main Results:

  • Various EM methods provide data on the three-dimensional structure and architecture of NPCs.
  • AFM allows for the investigation of the functional dynamics of native NPCs.
  • Combining techniques offers comprehensive insights into NPC organization.

Conclusions:

  • A spectrum of EM and AFM techniques are essential tools for elucidating NPC structure and function.
  • These advanced imaging methods provide critical information on nucleocytoplasmic transport mechanisms.