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T helper/inducer (CD4+) cells prestimulated with PPD induce monocytes to produce interleukin-1 beta
1Department of Medicine, University of Alabama, Birmingham 35294.
Abstract:
We obtained peripheral blood mononuclear cells (PBMC) from four healthy, tuberculin purified protein derivative (PPD) reactive donors and cultured these cells in media containing PPD (low dose = 200 ng/ml or high dose = 1 micrograms/ml). Five days after the addition of PPD, T cells were isolated, washed, and added to autologous adherent cell cultures at a 1:1 ratio. Adherent cells were then cultured for 24 h in media only (baseline), media plus lipopolysaccharide (LPS, 2 micrograms/ml; positive control), or media containing the prestimulated T cells. After 24 h, supernatants were harvested and interleukin 1 beta (IL-beta) levels were assayed by radioimmunoassay (RIA) or enzyme-linked immunosorbent assay (ELISA). The results show that T cells prestimulated with low dose PPD (200 micrograms/ml) did not induce IL-1 production by adherent cells (mean increase over baseline 0.2 +/- 1.3 standard deviation [SD] ng/ml, P = 0.61). However, T cells prestimulated with high dose PPD (1 microgram/ml) did induce adherent cells to secrete IL-1 beta (mean increase over baseline 1.7 +/- 0.62 [SD] ng/ml, P = 0.01), but this induction was abolished when cell-to-cell contact was prevented by use of double well chambers (mean increase over baseline 0.1 +/- 0.36 [SD] ng/ml, P = 0.69). Prestimulated T helper (CD4+) cells were able to induce monocytes to secrete IL-1 beta but prestimulated CD8+ T cells were not. These data suggest that when T helper (CD4+) cells are sufficiently activated they acquire the ability to induce monocytes to secrete IL-1 beta. Cell-to-cell contact between monocytes and T cells is required. This function of activated T cells may be important in the normal cellular immune response.
Insights
Activated T helper cells, but not CD8+ T cells, induce IL-1 beta secretion from monocytes. This requires cell-to-cell contact and is important for cellular immune responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- Interleukin-1 beta (IL-1 beta) is a key cytokine in inflammatory and immune responses.
- T cells play a crucial role in modulating monocyte function during immune responses.
Purpose of the Study:
- To investigate the role of T cell activation by purified protein derivative (PPD) in inducing IL-1 beta secretion from monocytes.
- To determine the specific T cell subsets (CD4+ vs. CD8+) involved in this process.
- To elucidate the requirement of cell-to-cell contact for T cell-mediated IL-1 beta induction.
Main Methods:
- Peripheral blood mononuclear cells (PBMC) from PPD-reactive donors were cultured with varying doses of PPD.
- T cells were isolated and co-cultured with autologous adherent cells (monocytes).
- IL-1 beta levels in supernatants were measured using radioimmunoassay (RIA) or enzyme-linked immunosorbent assay (ELISA).
Main Results:
- High-dose PPD prestimulated T cells induced significant IL-1 beta secretion from adherent cells.
- Low-dose PPD prestimulated T cells did not induce IL-1 beta secretion.
- T cell-mediated IL-1 beta induction was dependent on cell-to-cell contact.
- CD4+ T cells, but not CD8+ T cells, were responsible for inducing IL-1 beta secretion.
Conclusions:
- Sufficient activation of CD4+ T cells confers the ability to induce IL-1 beta secretion from monocytes.
- Cell-to-cell contact between T cells and monocytes is essential for this interaction.
- This T cell-monocyte crosstalk may be a significant mechanism in cellular immunity.