Characterization of the ends and target site of a novel tetracycline resistance-encoding conjugative transposon from
Adam P Roberts1, Ian J Davis, Lorna Seville
1Division of Microbial Diseases, UCL Eastman Dental Institute, University College London, University of London, 256 Gray's Inn Road, London WC1X 8LD, United Kingdom. aroberts@eastman.ucl.ac.uk
Abstract:
Enterococcus faecium 664.1H1 is multiply antibiotic resistant and mercury resistant. In this study, the genetic support for the tetracycline resistance of E. faecium 664.1H1 was characterized. The tet(S) gene is responsible for tetracycline resistance, and this gene is located on the chromosome of E. faecium 664.1H1, on a novel conjugative transposon. The element is transferable to Enterococcus faecalis, where it integrates into a specific site. The element was designated EfcTn1. The integrase of EfcTn1 is related to the integrase proteins found on staphylococcal pathogenicity islands. We show that the transposon is flanked by an 18-bp direct repeat, a copy of which is also present at the target site and at the joint of a circular form, and we propose a mechanism of insertion and excision.
More Related Videos
Related Concept Videos
Conjugation
Transposons
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...
Encoding
Automatic processing involves the encoding of details like time, space, frequency, and the meaning of words, usually done without conscious...
Conjugated Proteins
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Conjugated Proteins


