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Plasma adiponectin, T94G gene polymorphism and PAI-1 in patients with and without hypertension
1Division of Cardiology, Department of Internal Medicine, and Neuro-Medical Scientific Center, Buddhist Tzu-Chi General Hospital, Tzu-Chi University, Hualien, Taiwan, Republic of China.
Insights
The adiponectin T94G gene polymorphism is linked to lower adiponectin and higher plasminogen activator inhibitor-1 (PAI-1) levels in Chinese individuals. This genetic link may influence adiponectin and PAI-1 regulation, particularly in men and normotensive subjects.
Area of Science:
- Genetics
- Endocrinology
- Cardiovascular Disease
Background:
- Reduced adiponectin is linked to metabolic syndrome, type 2 diabetes, and coronary artery disease.
- The relationship between the adiponectin T94G genotype, plasma adiponectin, and plasminogen activator inhibitor-1 (PAI-1) is not well understood.
Purpose of the Study:
- To investigate the associations between the adiponectin T94G genotype, plasma adiponectin levels, PAI-1 levels, and blood pressure in a Chinese population.
Main Methods:
- Genotyping for the adiponectin T94G polymorphism was performed on 568 Chinese patients.
- Plasma levels of adiponectin and PAI-1 were measured.
- Patients were categorized into hypertensive and normotensive groups.
Main Results:
- Hypertensive patients had lower adiponectin and higher PAI-1 levels compared to normotensive subjects.
- Plasma adiponectin levels were inversely correlated with PAI-1 activity and antigen.
- The TT genotype group exhibited significantly lower plasma adiponectin and higher plasma PAI-1 activity and antigen compared to the GG genotype group.
Conclusions:
- The adiponectin T94G TT genotype is associated with lower plasma adiponectin and higher plasma PAI-1 levels in the Chinese population.
- This genotype's effect on adiponectin and PAI-1 appears more pronounced in men and normotensive individuals.
- The adiponectin gene T94G polymorphism may play a role in regulating circulating adiponectin and PAI-1 levels.
Background:
Reduced adiponectin level has been associated with metabolic syndrome, type 2 diabetes, coronary artery disease and gene polymorphisms, but the interrelationships of T94G genotype, plasma adiponectin and plasminogen activator inhibitor-1 (PAI-1) are less understood.
Patients And Methods:
The T94G genotypes and plasma levels of adiponectin, and PAI-1 were determined in 568 Chinese patients, 212 with and 356 without hypertension, to study the possible associations of T94G genotype, plasma adiponectin, PAI-1 and blood pressure.
Results:
Hypertensive patients showed significantly lower plasma adiponectin (9.7 +/- 11.1 vs. 11.5 +/- 10.0 microg/ml, p = 0.04) and higher PAI-1 (p < 0.001) levels but not significantly greater adiponectin TT genotype percentage (38.7 vs. 33.5%) and T allele frequency (0.620 vs. 0.585) than normotensive subjects. Plasma adiponectin was inversely related to PAI-1 activity (r = -0.09, p = 0.03) and antigen (r = -0.202, p < 0.001). Furthermore, the TT genotypic group showed significantly lower plasma adiponectin level (10.4 +/- 10.5 vs. 13.4 +/- 10.8 mug/ml, p = 0.03) and higher plasma PAI-1 activity (17.0 +/- 9.7 vs. 13.5 +/- 7.6 IU/ml, p = 0.003) and antigen (32.3 +/- 22.7 vs. 25.9 +/- 14.7 ng/ml, p = 0.01) than the GG genotypic group. Multiple linear regression analysis in all study subjects, in men and in normotensives documented an impact of adiponectin T94G genotype on plasma levels of adiponectin (p = 0.007, 0.003 and 0.03) and PAI-1 activity (p = 0.02, 0.03 and 0.04) and antigen (p = 0.03, 0.007 and 0.04) after adjustment for potential confounding factors.
Conclusions:
The present study demonstrated a significant correlation of the TT genotype with lower plasma adiponectin and higher plasma PAI-1 levels in a Chinese population. The contribution of this genotype seemed greater in men and normotensives. It suggested the adiponectin gene T94G polymorphism might affect the regulation of circulating adiponectin and PAI-1.
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