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Published on: December 30, 2025
The DU145 human prostate carcinoma cell line harbors a temperature-sensitive allele of p53
Marcio C Bajgelman1, Bryan E Strauss
1Viral Vector Group, Laboratory of Genetics and Molecular Cardiology, InCor, University of São Paulo School of Medicine, Brazil.
Background:
Some nuances of mutant and wild-type p53 activity have been uncovered utilizing temperature sensitive (TS) alleles. However, few human tumor derived cell lines possess a TS p53 mutant.
Methods:
The cell lines DU145 (heterozygous p53, P223L, and V274F) and PC3 (p53-null, where exogenous P223L and V274F were introduced individually or in combination) were examined for TS p53 activity as revealed by reporter construct and target gene activation.
Results:
TS p53 function was observed in DU145 and expression of the P223L allele in PC3 conferred a TS p53 profile. Activation of p21Waf1 demonstrated that P223L TS activity may have been influenced by cellular context.
Conclusions:
The DU145 cell line harbors a TS mutant of p53 and, in addition to being a widely used model of human prostate carcinoma, may also reveal new insights into p53 function due to the unique transcriptional properties of its TS phenotype.
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