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Reaction of dipeptidylpeptidase IV with substrate-analogous azapeptides
U Neumann1, T Steinmetzer, A Barth
1Institute of Pharmacology and Toxicology, Medical Academy Erfurt, GDR.
Journal of Enzyme Inhibition
|January 1, 1991
Abstract:
The reaction of dipeptidyl peptidase IV (EC 3.4.14.5.) with azapeptide substrates containing azaalanine or azaproline in the P1-position was investigated. Accumulation of a fairly stable acyl-enzyme could be shown for ester substrates. Ala-AzaPro-pNA is a very poor substrate of DP IV and does not accumulate an acyl-enzyme. DP IV does not react with active-site titrants for trypsin-like serine proteases.