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A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
Published on: May 18, 2013
Blood storage at 4 degrees C-factors involved in DNA yield and quality
Andrea J Richardson1, Niro Narendran, Robyn H Guymer
1Centre for Eye Research Australia, University of Melbourne, East Melbourne, Australia. andreajr@unimelb.edu.au
Background:
Whole blood provides one of the most common sources of both high-quality DNA and high-quantity DNA for molecular biological purposes. Typically, blood storage at 4 degrees C is short term, which ranges from a few days to a few weeks. However, long-term storage usually involves blood being frozen, with a resultant loss in DNA yield. The authors examined the effects of long-term storage at 4 degrees C.
Methods:
Duplicate blood samples were collected from 301 participants (aged 20-98 years) enrolled as part of ongoing studies. Samples were stored at 4 degrees C for between 11 days and 922 days, and DNA was subsequently extracted using a phenol/chloroform procedure.
Results:
A negative correlation of the number of storage days existed at 4 degrees C with DNA yield. The main determinant on DNA yield was the age of the participant in the study, with older persons having a lower DNA yield.
Conclusions:
Long-term storage of blood at 4 degrees C does have a detrimental effect on DNA yield, but this effect seemed less significant than the age of a person. The impact of age of a person or storage time has a minimal impact on DNA quality. Therefore, storage of blood at 4 degrees C offers an acceptable alternative to frozen storage.
