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Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
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Chronic urticaria sera increase basophil CD203c expression.

Karen M Yasnowsky1, Stephen C Dreskin, Benjamin Efaw

  • 1Department of Medicine, Division of Allergy and Clinical Immunology, University of Colorado Health Sciences Center and the National Jewish Medical and Research Center, Denver, CO 80206, USA.

The Journal of Allergy and Clinical Immunology
|June 6, 2006
PubMed
Summary
This summary is machine-generated.

Sera from chronic idiopathic urticaria patients significantly increase basophil CD203c expression, a marker linked to histamine release. This finding may help identify patients with this condition.

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Area of Science:

  • Immunology
  • Allergy Research
  • Dermatology

Background:

  • Chronic idiopathic urticaria (CIU) affects many patients, with ~40% having antibodies against the high-affinity IgE receptor.
  • CD203c, a basophil activation marker, is upregulated by FcepsilonRIalpha receptor cross-linking and may identify CIU patients.

Purpose of the Study:

  • To evaluate the effect of CIU patient sera on basophil CD203c expression.
  • To correlate CD203c levels with histamine release and autologous serum skin test (ASST) size.
  • To investigate if IgG antibodies mediate this effect.

Main Methods:

  • Sera from CIU patients (ASST+ or ASST-) and controls were incubated with donor whole blood.
  • Basophil activation was measured via flow cytometry, identifying CD203c on IgE-positive cells.

Main Results:

  • Sera from ASST+ CIU patients significantly upregulated CD203c on basophils.
  • IgG depletion reduced CD203c upregulation, indicating an IgG-mediated mechanism.
  • CD203c expression correlated with histamine release and ASST size.

Conclusions:

  • CIU patient sera, particularly from ASST+ individuals, significantly increase basophil CD203c expression.
  • This upregulation correlates with basophil histamine release, suggesting CD203c as a potential biomarker in CIU.