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Updated: Aug 8, 2026

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Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
Published on: April 2, 2016
Deletion polymorphism of Disc1 is common to all 129 mouse substrains: implications for gene-targeting studies of
Steven J Clapcote1, John C Roder
1Mount Sinai Hospital Research Institute, Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada. clapcote@mshri.on.ca
Genetics
|June 6, 2006
Abstract:
We report that the Disc1 gene in all extant 129 mouse inbred substrains has a deletion, previously considered specific to the 129S6/SvEv substrain, which is predicted to abolish production of the full-length protein. This finding has implications for the study of knockout mice generated from 129-derived embryonic stem cells.

