Related Experiment Video

Updated: Aug 8, 2026

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
08:04

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Published on: March 18, 2014

Adenovirus-mediated p53 tumor suppressor gene therapy of osteosarcoma

Vladimir V Ternovoi1, David T Curiel, Bruce F Smith

  • 1Division of Human Gene Therapy, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35233, USA.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|June 6, 2006
PubMed

Insights

Gene therapy using p53, a tumor suppressor, shows promise for osteosarcoma (OS) treatment. Current adenovirus vectors need improvement for better efficacy in clinical applications against this challenging cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Therapy

Background:

  • Osteosarcoma (OS) treatment outcomes remain poor despite conventional therapies.
  • Tumor suppressor gene therapy, specifically p53 gene replacement, offers a novel therapeutic avenue.
  • Previous p53 gene therapy trials faced challenges with vector efficacy and in vivo activity.

Purpose of the Study:

  • To review the current status of adenovirus-mediated p53 gene therapy for osteosarcoma.
  • To discuss advancements in understanding p53's role and chemoresistant OS biology.
  • To explore improved tumor targeting strategies using adenoviral vectors.

Main Methods:

  • Review of existing literature on p53 gene therapy for osteosarcoma.
  • Analysis of clinical trial data regarding vector efficacy and p53 activity.
  • Examination of molecular mechanisms in chemoresistant OS and adenoviral vector technology.

Main Results:

  • Wild-type p53 introduction can be lethal to cancer cells in vitro.
  • Clinical trials demonstrated limited success due to insufficient vector efficacy and low in vivo proapoptotic activity.
  • Recent advancements offer potential for enhanced tumor targeting and therapeutic outcomes.

Conclusions:

  • Adenovirus-mediated p53 gene therapy holds potential for osteosarcoma treatment.
  • Further research is needed to optimize vectors and enhance p53's therapeutic efficacy.
  • Future investigations should focus on improving tumor targeting and overcoming chemoresistance.

More Related Videos

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Cancer Therapies02:49

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...