Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cox7a2 mediates steroidogenesis in TM3 mouse Leydig cells.

Liang Chen1, Zhong-Cheng Xin, Xin Li

  • 1Andrology Center of Peking University First Hospital, Beijing 100009, China.

Asian Journal of Andrology
|June 6, 2006
PubMed
Summary

Cox7a2 inhibits testosterone production in mouse Leydig cells by reducing steroidogenic acute regulatory (StAR) protein expression. This effect is mediated by increased reactive oxygen species (ROS) activity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Effects of Pa-pex 11 gene on penicillin production in Penicillium aurantiogriseum].

Wei sheng wu xue bao = Acta microbiologica Sinica·2010
Same author

Danggui-Shaoyao-San and its active fraction JD-30 improve Abeta-induced spatial recognition deficits in mice.

Journal of ethnopharmacology·2010
Same author

Inhibition of lung fluid clearance and epithelial Na+ channels by chlorine, hypochlorous acid, and chloramines.

The Journal of biological chemistry·2010
Same author

Discovery and optimization of novel 3-piperazinylcoumarin antagonist of chemokine-like factor 1 with oral antiasthma activity in mice.

Journal of medicinal chemistry·2010
Same author

Evidence for dimeric BACE-mediated APP processing.

Biochemical and biophysical research communications·2010
Same author

Involvement of mineralocorticoid receptor in high glucose-induced big mitogen-activated protein kinase 1 activation and mesangial cell proliferation.

Journal of hypertension·2010

Area of Science:

  • Mitochondrial biology
  • Steroidogenesis regulation
  • Cellular signaling

Background:

  • Cytochrome c oxidase subunit 7A2 (Cox7a2) is a component of the mitochondrial respiratory chain.
  • Leydig cells are crucial for testosterone synthesis in the testes.
  • The regulation of steroidogenesis involves complex cellular mechanisms.

Purpose of the Study:

  • To elucidate the role of Cox7a2 in regulating steroidogenesis in TM3 mouse Leydig cells.
  • To investigate the underlying molecular mechanisms, including the involvement of StAR protein and ROS.

Main Methods:

  • Cloning and overexpression of Cox7a2 in TM3 mouse Leydig cells.
  • Measurement of testosterone secretion using ELISA.
  • Assessment of steroidogenic acute regulatory (StAR) protein expression via Western blot.

Related Experiment Videos

  • Quantification of reactive oxygen species (ROS) activity using fluorometry.

    • Main Results:

    • Overexpression of Cox7a2 significantly inhibited luteinizing hormone (LH)-induced testosterone secretion.
    • Cox7a2 overexpression led to decreased expression of StAR protein.
    • Increased reactive oxygen species (ROS) activity was observed in Cox7a2-overexpressing cells.

    Conclusions:

    • Cox7a2 negatively regulates steroidogenesis in mouse Leydig cells.
    • The inhibitory effect of Cox7a2 on testosterone production is, at least partly, mediated by increased ROS activity, leading to reduced StAR protein expression.