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Related Experiment Videos

Encapsulation of BSA using a modified W/O/O emulsion solvent removal method.

Deborah McK Ciombor1, Ana Jaklenec, Alex Z Liu

  • 1Department of Orthopaedics, Brown Medical School, Rhode Island Hospital, Providence, RI 02912, USA.

Journal of Microencapsulation
|June 7, 2006
PubMed
Summary

This study optimized protein encapsulation in polylactic-co-glycolic-acid (PLGA) microspheres using a w/o/o emulsion method. High yields and controlled release were achieved by adjusting suspension medium and phase ratios.

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Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Polymer Chemistry

Background:

  • Protein encapsulation in biodegradable polymers is crucial for controlled drug release.
  • Polylactic-co-glycolic-acid (PLGA) is a widely used biomaterial for drug delivery applications.
  • Optimizing encapsulation efficiency and controlling release kinetics remain key challenges.

Purpose of the Study:

  • To systematically investigate and optimize protein encapsulation within PLGA microspheres.
  • To evaluate the impact of various formulation parameters on microsphere fabrication and encapsulation efficiency.
  • To establish a method for controlling protein release rates through microsphere porosity modulation.

Main Methods:

  • Utilized a water-in-oil-in-oil (w/o/o) emulsion technique followed by solvent removal for microsphere fabrication.

Related Experiment Videos

  • Investigated the effects of suspension medium composition (e.g., silicon oil with methylene chloride vs. pure silicon oil).
  • Examined the influence of aqueous phase volume to polymer solution ratios and polymer concentrations (2-15% g ml-1).

    • Main Results:

    • Achieved high yields of microsphere fabrication (>98%) with minimal polymer and protein loss (<2%).
    • Identified optimal conditions for successful encapsulation, requiring aqueous phase to polymer solution volume ratios <5% (v/v).
    • Demonstrated that the w/o/o emulsion method facilitates control over protein release rates by modulating microsphere porosity.

    Conclusions:

    • The w/o/o emulsion and solvent removal method is highly effective for high-yield protein encapsulation in PLGA microspheres.
    • Formulation parameters, particularly the suspension medium and phase ratios, significantly impact encapsulation efficiency and yield.
    • This approach offers a versatile platform for developing protein-loaded PLGA microspheres with tunable release characteristics.