[Influence of captopril on human sperm motility parameters in vitro]
1Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan, Hubei 430030, China.
Objective:
To determine the effects of captopril on human sperm motility in vitro.
Methods:
Sperm specimens were aseptically obtained by masturbation and prepared by Percoll gradient-centrifugation technique to produce a spermatozoa suspension of high motility from 12 healthy fertile men. The spermatozoa suspension were incubated with captopril at 1:9 ratio of volume at 37 degrees C in vitro, which was administrated at three different concentrations. Measurements were carried out within 5 minutes for all specimens including VAP, VSL, VCL, STR, ALH and progressive motility.
Results:
Within 5 minutes, three different concentrations of captopril could change the human spermatozoa motility and forward motile sperm percentage, but captopril did not decrease the other motility parameters, including VSL, VCL and VAP etc. The high concentration group could decrease the VCL more than those of the other two groups and it seemed that the velocity parameters of the middle concentration group were higher than those of the other two groups, but there were no significant difference statistically.
Conclusion:
It suggests that captopril can decrease the human spermatozoa motility and forward motile sperm percentage, but it can not directly affect the spermatozoa velocity in vitro.
Insights
Captopril negatively impacts human sperm motility and forward movement in vitro. However, it does not directly alter sperm velocity parameters like VSL, VCL, or VAP.
Area of Science:
- Reproductive biology
- Pharmacology
- Andrology
Context:
- Sperm motility is crucial for male fertility.
- Angiotensin-converting enzyme (ACE) inhibitors, like captopril, are widely used to treat hypertension.
- Potential effects of common medications on sperm parameters require investigation.
Purpose:
- To investigate the in vitro effects of captopril on human sperm motility.
- To assess the impact of varying captopril concentrations on sperm functional parameters.
Summary:
- Human spermatozoa suspensions were incubated with captopril at three different concentrations.
- Sperm motility parameters, including VAP, VSL, VCL, STR, ALH, and progressive motility, were measured within 5 minutes.
- Captopril significantly reduced overall sperm motility and forward motile sperm percentage, but did not directly affect velocity parameters (VSL, VCL, VAP).
Impact:
- Captopril exposure in vitro can impair human sperm motility and forward progression.
- The findings suggest a potential, though not fully elucidated, impact of captopril on sperm function relevant to fertility.
- Further research is needed to understand the clinical implications of these in vitro observations.
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