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Related Experiment Videos

Fluorescent nanoparticle uptake for brain tumor visualization.

Rachel Tréhin1, Jose-Luiz Figueiredo, Mikael J Pittet

  • 1Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

Neoplasia (New York, N.Y.)
|June 8, 2006
PubMed
Summary
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Multimodal nanoparticles accurately estimate brain tumor margins by detecting both tumor and host cells. This imaging approach shows promise for improving surgical resection in various brain tumor models.

Area of Science:

  • Neuro-oncology
  • Nanomedicine
  • Surgical Oncology

Background:

  • Accurate delineation of brain tumor margins is critical for successful surgical resection.
  • Multimodal nanoparticles (CLIO-Cy5.5) have been developed for intraoperative visualization using MRI and fluorescence.

Purpose of the Study:

  • To evaluate the accuracy of fluorescent nanoparticle-based tumor margin determination in orthotopic brain tumor models with varying host immune responses.
  • To investigate nanoparticle uptake by tumor cells and host immune cells at the tumor margin.

Main Methods:

  • Orthotopic 9L gliosarcoma (GFP-expressing) tumors were implanted in nude mice and rats.
  • Fluorescent micrographs and flow cytometry were used to analyze nanoparticle (Cy5.5) uptake in tumor and host cells (CD11b+).

Related Experiment Videos

  • Tumor margins were compared between Cy5.5 fluorescence and GFP fluorescence.

    • Main Results:

    • Mean overestimations of tumor margins were 2 µm (nude mice) and 24 µm (rats) using Cy5.5 compared to GFP.
    • Nanoparticle uptake was confirmed in both tumor cells and CD11b+ cells (microglia/macrophages) in both models.
    • CD11b+ cells were concentrated at the tumor margin, particularly in rats.
    • Similar uptake patterns were observed in metastatic (CT26 colon) and primary (Gli36 glioma) brain tumor models.

    Conclusions:

    • Fluorescent nanoparticles provide accurate brain tumor margin estimation through combined tumor and host cell uptake.
    • This method is effective in both primary and metastatic brain tumor models.
    • The findings support the potential clinical translation of fluorescent nanoparticles for image-guided surgery.