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Updated: Aug 8, 2026

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
Published on: February 21, 2018
Resistance to phorbol ester-induced differentiation in human myeloid leukemia cells: a hypothetic role for the mRNA
Pierre Champelovier1, Virginie Pautre, Michèle Elatifi
1Laboratoire de Dynamique Cellulaire de l'EPHE, UMR CNRS 5525, Université Joseph Fourier, 38000 Grenoble, France.
Abstract:
UM384 cells, derived from the human myeloid leukemia U937 cell line, fail to differentiate in response to 12-O-tetradecanoylphorbol-13-acetate (TPA). Using cDNA microarray and real-time quantitative PCR (RT-QPCR) approaches, we observed a difference in the response to TPA treatment: all the genes from U937 cells were continuously modulated from 2 to 24h. In UM384 cells, 60% of the genes were transiently modulated at 2h, then returned to control levels at 24h. Moreover, HuR, an AU-rich element-binding protein (ARE-BP), was differentially located in the two cell lines. Therefore, a defect of mRNA stabilization could be responsible for the resistance of UM384 cells to TPA-induced differentiation, suggesting a possible role for the post-transcriptional regulation in the leukemogenesis.
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