Related Experiment Video
Updated: Aug 8, 2026

Extraction and Characterization of Surfactants from Atmospheric Aerosols
Published on: April 21, 2017
Spectrophotometric method for quantitative determination of nonionic, ionic and zwitterionic detergents
Sona Rajakumari1, Malathi Srinivasan, Ram Rajasekharan
1Lipid Laboratory, Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Abstract:
Detergents serve as means of solubilizing biological membranes and thus play an important role in purification and characterization of membrane proteins. We report here a simple method to estimate the amount of detergent bound to a protein or present in an aqueous solution. The method is based on the turbidity caused by the addition of a detergent to triolein. Detergent bound to an integral membrane protein, lysophosphatidic acid acyltransferase, was separated by native gel electrophoresis and the amount of detergent bound to the same was estimated. This method is applicable for Triton X-100, sodium dodecyl sulfate and zwitterionic detergent, and was validated in the presence of reagents commonly used in membrane protein solubilization and purification.
More Related Videos
Related Concept Videos
Detergent Purification of Membrane Proteins
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Qualitative Analysis
For instance, group IV...
High-Performance Liquid Chromatography: Types of Detectors
EDTA: Indirect and Alkalimetric Titration

