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Updated: Aug 8, 2026
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Evaluation of a real-time nucleic acid sequence-based amplification assay using molecular beacons for detection of
D R McClernon1, C Vavro, M St Clair
1Clinical Virology Department, GlaxoSmithKline, Research Triangle Park, NC 27709-3398, USA. daniel.r.mcclernon@gsk.com
Abstract:
We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially available assays. The analytical sensitivity was 37 IU/ml. The NASBA assay detected clinically relevant recombinant viruses and all group M HIV-1 subtypes.
