Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RACK1, a novel hPER1-interacting protein.

Lijuan Hu1, Fang Lu, Yuhui Wang

  • 1West China Medical Center, Sichuan University, Chengdu, Sichuan 610041, P. R. China.

Journal of Molecular Neuroscience : MN
|June 8, 2006
PubMed
Summary

Researchers discovered Receptor for Activated Protein Kinase C-1 (RACK1) interacts with the human Period 1 (hPER1) clock protein. This protein interaction suggests RACK1 may regulate circadian rhythm functions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulation of Intrinsic and Extrinsic Apoptotic Pathways in Osteosarcoma Cells Following Oleandrin Treatment.

International journal of molecular sciences·2016
Same author

Reinnervated Split-Muscle Technique for Creating Additional Myoelectric Sites in an Animal Model.

Plastic and reconstructive surgery·2016
Same author

Graphene oxide-based efficient and scalable solar desalination under one sun with a confined 2D water path.

Proceedings of the National Academy of Sciences of the United States of America·2016
Same author

Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration.

Stem cell research & therapy·2016
Same author

Nanotechnology Based Green Energy Conversion Devices with Multifunctional Materials at Low Temperatures.

Recent patents on nanotechnology·2016
Same author

State-Dependent Allosteric Inhibition of the Human SLC13A5 Citrate Transporter by Hydroxysuccinic Acids, PF-06649298 and PF-06761281.

Molecular pharmacology·2016

Area of Science:

  • Molecular Biology
  • Chronobiology
  • Cell Signaling

Background:

  • Period 1 (PER1) is crucial for circadian rhythm stability.
  • Receptor for Activated Protein Kinase C-1 (RACK1) is a versatile scaffolding protein involved in signal transduction.

Purpose of the Study:

  • To identify novel protein interactions with human PER1 (hPER1).
  • To investigate the role of RACK1 in relation to hPER1 function.

Main Methods:

  • Yeast two-hybrid system for protein interaction screening.
  • Co-immunoprecipitation to confirm hPER1-RACK1 interaction.
  • RT-PCR to analyze RACK1 expression patterns.

Main Results:

  • RACK1 was identified as a novel interacting protein of hPER1.

Related Experiment Videos

  • RACK1 exhibits widespread tissue expression without significant rhythmicity.
  • Inhibition of hPER1 did not affect RACK1 expression, indicating a unidirectional interaction.

    • Conclusions:

    • RACK1 interacts with hPER1, suggesting a role in regulating circadian clock mechanisms.
    • RACK1 may function as a novel signaling molecule mediating hPER1 activity.
    • The interaction highlights a new pathway for circadian rhythm regulation.