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Cornea engineering on polyester carriers.

P Zorlutuna1, A Tezcaner, I Kiyat

  • 1Department of Biological Sciences, Biotechnology Research Unit, Middle East Technical University, Ankara 06531, Turkey.

Journal of Biomedical Materials Research. Part A
|June 8, 2006
PubMed
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Biodegradable polyester carriers made from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(L/DL-lactide) (P(L/DL)LA) blends show potential for cornea tissue engineering. These materials successfully supported epithelial and stromal cell growth in vitro.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Ophthalmology

Background:

  • Corneal tissue engineering aims to regenerate damaged corneal layers.
  • Biodegradable polyesters offer promising scaffolds for cell growth and tissue regeneration.

Purpose of the Study:

  • To design and evaluate biodegradable polyester-based carriers for corneal epithelial and stromal tissue engineering.
  • To assess the in vitro performance of these carriers seeded with relevant cell types.

Main Methods:

  • Micropatterned films of PHBV/P(L/DL)LA blends coated with fibronectin were used for the epithelial layer and seeded with D407 cells.
  • Highly porous foams of PHBV/P(L/DL)LA blends were used for the stromal layer and seeded with 3T3 fibroblasts.
  • Cell proliferation, morphology, phenotype retention, and extracellular matrix deposition were analyzed using SEM and immunohistochemistry.

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Main Results:

  • Polyester carriers supported significantly higher cell numbers compared to polystyrene controls.
  • D407 cells formed multilayers and retained epithelial phenotype with tight junction formation.
  • 3T3 fibroblasts showed homogeneous distribution and deposited collagen type I, though core cell population was limited in thick foams.
  • SEM confirmed porous and interconnected foam structures.

Conclusions:

  • PHBV/P(L/DL)LA blends are suitable for creating micropatterned films and porous foams for corneal tissue engineering.
  • These materials demonstrate significant potential for reconstructing both epithelial and stromal corneal layers.
  • Further optimization may be needed to enhance cell penetration in thicker stromal scaffolds.