Related Experiment Video
Updated: Aug 8, 2026

Cell Subtype-specific Analysis of Neuronal Membrane Proteasome in Somatosensory Neurons
Published on: October 10, 2025
Localization of calcium-activated neutral protease (CANP) in the peripheral nerve
K Kamakura1, S Ishiura, T Fujita
1Division of Neuromuscular Research, National Center for Nervous, Mental and Muscular Disorders, Kodaira, Tokyo, Japan.
Abstract:
Localization of calcium-activated neutral protease (CANP) in the rat peripheral nerve was studied by an indirect immunofluorescent method, using rabbit antiserum against CANP extracted from chicken skeletal muscle. Its specificity to CANP, as well as its cross-reactivity with rat CANP, were confirmed by the Ouchterlony immunodiffusion procedure and immunoreplica method. CANP was demonstrated in the axon, but not in the myelin sheath or endoneurium. We could not conclude whether or not CANP in axons exists in association with neurofilaments.
More Related Videos
Related Concept Videos
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Nociception
Local Anesthetics: Mechanism of Action
Local anesthetics are amphiphilic molecules consisting of a hydrophobic aromatic part linked to a hydrophilic group by an ester or amide linkage. They are weak bases and are usually available as salts, which increases their solubility and stability. Once administered, LAs exist in the body either...
Local Anesthetics: Differential Sensitivity of Nerve Fibers
Ligand-Gated Ion Channel Receptor: Gating Mechanism

