Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Germ cell-less expression in zebrafish embryos.

Wenyan Li1, Fengjiao Deng, Huannan Wang

  • 1Key Laboratory of MOE for Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China.

Development, Growth & Differentiation
|June 9, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alterations in Metabolites Associated With Umbilical Cord Blood in Monozygotic Twins Discordant for Congenital Heart Disease.

Pediatric discovery·2026
Same author

A Green UPLC-UV Method for L-Ascorbic Acid Determination Based on a Biodegradable Chelating Agent and Synergistic Hydrophobic-Electrostatic Interactions.

Journal of AOAC International·2026
Same author

Liensinine Ameliorating Oxidative Damage to Vascular Endothelium In Vitro via Upregulating eNOS and Nrf2/HO-1 Signaling.

Food science & nutrition·2026
Same author

Echoes of Trauma: Multi-Timescale Pathways Linking Childhood Trauma to Adolescent Non-Suicidal Self-Injury via Social Exclusion.

Suicide & life-threatening behavior·2026
Same author

Surface-displayed multivalent PRRSV tandem antigen on Bacillus subtilis spores induces mucosal immune responses in mice.

Journal of applied microbiology·2026
Same author

Kaempferol protects melanocytes from ferroptosis by modulating the NF-κB/PTGS2 signaling axis in vitiligo.

Apoptosis : an international journal on programmed cell death·2026
Same journal

Atypical Cadherin Fat2 is Involved in Axogenesis of Cerebellar Granule Cells in Zebrafish.

Development, growth & differentiation·2026
Same journal

Sbno1 and Usp8 Cooperate to Enhance Notch Signaling in Regulating Neural Stem Cells.

Development, growth & differentiation·2026
Same journal

Computer-Aided Sperm Analysis Protocol for Evaluating Sperm Motility in Japanese Medaka.

Development, growth & differentiation·2026
Same journal

Cooperative Roles of Pds5a and Pds5b Constrain Long-Range Chromatin Interactions in Vertebrate Embryos.

Development, growth & differentiation·2026
Same journal

Derepression of a Subset of Meiotic Proteins in Primordial Germ Cells of max Mutant Zebrafish.

Development, growth & differentiation·2026
Same journal

Convergence of Cell Biology and Developmental Biology: A Report of the Joint Meeting of the 77th JSCB and the 58th JSDB.

Development, growth & differentiation·2026
See all related articles

Zebrafish germ cell-less (gcl) gene expression changes during early development, with transcripts localizing to future gonad cells. This suggests gcl is vital for primordial germ cell formation in zebrafish.

Area of Science:

  • Developmental Biology
  • Genetics
  • Zebrafish Research

Background:

  • The germ cell-less (gcl) gene is crucial for early germ cell differentiation in Drosophila.
  • While gcl homologs exist, their expression and function in zebrafish remain largely uncharacterized.

Purpose of the Study:

  • To investigate the expression pattern of the zebrafish gcl gene during embryonic development.
  • To analyze the potential role of zebrafish gcl in primordial germ cell formation.

Main Methods:

  • Real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) for mRNA expression levels.
  • Whole-mount in situ hybridization (WISH) to visualize transcript localization during development.

Main Results:

Related Experiment Videos

  • Zebrafish gcl mRNA levels decrease significantly from the 4-cell to sphere stage, then increase and stabilize.
  • gcl transcripts are initially ubiquitous, then localize to blastoderm margin clusters during gastrulation.
  • Subcellular clumps of gcl mRNA are found in cells migrating to the dorsal gonad position and are widely expressed in adult germ cells.

    • Conclusions:

    • Zebrafish gcl exhibits dynamic spatiotemporal expression during embryogenesis.
    • The localization patterns suggest a critical role for gcl in primordial germ cell development and migration.