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Updated: Aug 7, 2026

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
Published on: June 25, 2014
An improved PCR-based amplification of unknown homologous DNA sequences
Fakhraddin Naghibalhossaini1, Afsaneh Moaddeb
1Department of Biochemistry, Shiraz University of Medical Sciences, Zand Street, Shiraz 71345, Iran. fakhraddin.naghibalhossaini@elf.mcgill.ca
Abstract:
The PCR primers used for cloning of evolutionary conserved genes or homologous DNA sequences are usually guessmer oligonucleotides. We introduce a simple way using Pfu polymerase to overcome possible PCR amplification failure because of 3'-end mismatches of guessed primers with the target DNA.
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