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Updated: Aug 7, 2026

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
Published on: October 17, 2018
Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different
Marcin Moniuszko1, Krzysztof Kowal, Malgorzata Rusak
1Department of Allergology and Internal Medicine, Medical University of Bialystok, Sklodowskiej-Curie Street 24A, 15-276 Bialystok, Poland. moniuszm@amb.edu.pl
We investigated whether the choice of anticoagulant or the application of density gradient mononuclear cell isolation may account for conflicting published data regarding the levels of the scavenger receptors' expression in healthy individuals. We demonstrate that the detection of CD163, but not CD36, differs dramatically among the methods.
We investigated whether the choice of anticoagulant or the application of density gradient mononuclear cell isolation may account for conflicting published data regarding the levels of the scavenger receptors' expression in healthy individuals. We demonstrate that the detection of CD163, but not CD36, differs dramatically among the methods.

