Related Experiment Video

Updated: Aug 7, 2026

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
11:17

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Published on: March 10, 2021

Matrix protein and glycoproteins F and H of Peste-des-petits-ruminants virus function better as a homologous complex

M Mahapatra1, S Parida1, M D Baron1

  • 1Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, UK.

The Journal of General Virology
|June 9, 2006
PubMed

Insights

Developing a new peste des petits ruminants (PPR) vaccine, RPV-PPRMFH, improved viral growth and goat protection. This triple chimera shows promise as a marker vaccine candidate for PPR disease control.

Area of Science:

  • Virology
  • Immunology
  • Veterinary Medicine

Background:

  • Paramyxovirus matrix (M) protein is crucial for viral structure and assembly.
  • Previous chimeric vaccines (RPV-PPRFH) for peste des petits ruminants (PPR) exhibited poor tissue culture growth.
  • M protein interactions with surface glycoproteins were hypothesized to cause poor growth in RPV-PPRFH.

Purpose of the Study:

  • To improve the growth and efficacy of a peste des petits ruminants (PPR) marker vaccine.
  • To investigate the role of the matrix (M) protein in chimeric virus replication.
  • To develop a more effective vaccine candidate for PPR disease.

Main Methods:

  • Construction of a triple chimeric virus (RPV-PPRMFH) by replacing M, F, and H genes of Rinderpest virus (RPV) with those from Peste-des-petits-ruminants virus (PPRV).
  • Evaluation of viral growth in tissue culture.
  • Assessment of vaccine efficacy in goats, including protection against wild-type PPRV challenge and antibody response.

Main Results:

  • The triple chimera (RPV-PPRMFH) demonstrated improved growth in tissue culture compared to the RPV-PPRFH chimera.
  • RPV-PPRMFH grew to titers comparable to unmodified PPRV, though lower than parental RPV.
  • Goats vaccinated with RPV-PPRMFH showed no adverse effects and were protected from PPRV challenge, with significantly higher neutralizing antibody titers.

Conclusions:

  • Replacing M, F, and H genes of RPV with PPRV counterparts enhances chimeric virus growth.
  • The triple chimera RPV-PPRMFH is a promising candidate for a peste des petits ruminants (PPR) marker vaccine.
  • RPV-PPRMFH elicits a robust immune response and provides protection against PPRV infection.

More Related Videos

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses08:10

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Published on: January 15, 2020

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy09:13

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Globular Proteins01:27

Globular Proteins

In organisms, proteins are the most abundant macromolecules. They act as the building blocks of life and play various crucial roles in the body. Proteins can be broadly classified into two distinct subtypes based on their shape and solubilities: globular proteins and fibrous proteins.
Globular proteins serve many important physiological functions, such as acting as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be soluble in the aqueous...