Analytical method for determining iminoctadine triacetate by LC/ESI/MS using hydrophilic interaction chromatography

Area of Science:

• Environmental Chemistry
• Analytical Chemistry
• Food Safety

Background:

• Japan established a target value of 6 microg/l for iminoctadine triacetate residues in tap water.
• Accurate quantification of iminoctadine triacetate is crucial for ensuring water safety.

Purpose of the Study:

• To develop a highly selective and sensitive analytical method for iminoctadine triacetate.
• To validate the method's performance in various water matrices.

Main Methods:

• Solid phase extraction (SPE) coupled with liquid chromatography-electrospray ionization-mass spectrometry (LC/ESI/MS).
• Utilized hydrophilic interaction chromatography (HILIC) for enhanced separation.
• Analyzed iminoctadine triacetate in distilled water, tap water, and raw water.

Main Results:

• Achieved high recovery rates ranging from 77.1% to 96.7% at different concentrations (0.06, 0.6, and 6 microg/l).
• Demonstrated good precision with coefficients of variation (CV) between 3.7% and 13.2%.
• Established a quantitation limit of 0.04 microg/l, significantly lower than the target value.

Conclusions:

• The developed LC/ESI/MS method is highly selective and sensitive for iminoctadine triacetate determination.
• The method is suitable for routine monitoring of iminoctadine triacetate in various water types.
• Quantification at one-hundredth of the target value confirms the method's capability for stringent water quality control.