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Optic nerve alterations in PTEN +/- mice.

E Lopez-Sanchez1, E Frances-Muñoz, J L Menezo

  • 1Hospital Arnau de Vilanova, Valencia--Spain. elopsan@ono.com

European Journal of Ophthalmology
|June 9, 2006
PubMed
Summary

PTEN gene knockout in mice resulted in larger optic nerves (ON) with hypertrophic axons. However, these PTEN-gene knockout mice showed no significant changes in axon count or myelin sheath structure.

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Area of Science:

  • Neuroscience
  • Genetics
  • Ophthalmology

Background:

  • The PTEN gene plays a crucial role in regulating cell size, cell cycle arrest, and apoptosis.
  • Understanding PTEN's function is vital for neurological and ophthalmological research.

Purpose of the Study:

  • To investigate the impact of PTEN gene knockout on the morphologic characteristics of the optic nerve (ON).
  • To utilize a mouse model with altered PTEN expression to study ON development and structure.

Main Methods:

  • Morphologic and morphometric analyses were performed on optic nerves from wild-type and heterozygous PTEN knockout mice.
  • Light and electron transmission microscopy were employed to examine ON ultrastructure.
  • Western blot and immunoblotting were used to assess glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) expression.

Main Results:

  • Optic nerves in PTEN knockout mice exhibited a significantly larger cross-sectional area compared to wild-type controls.
  • Axons in PTEN knockout mice were significantly larger than those in wild-type mice.
  • No significant differences were observed in axon number, intra-axonal degeneration, myelin sheath integrity, or axoplasm density.

Conclusions:

  • Heterozygous PTEN knockout mice display hypertrophic optic nerves.
  • These morphologic changes occur without detectable ultrastructural alterations in the optic nerve.

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