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Related Experiment Videos

Runx3 expression during mouse tongue and palate development.

Hiromitsu Yamamoto1, Kosei Ito, Mariko Kawai

  • 1Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan. hyamamot@kuhp.kyoto-u.ac.jp

The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology
|June 9, 2006
PubMed
Summary

Transforming growth factor beta (TGF-beta) and bone morphologic protein (BMP) signaling pathways are crucial for mammalian oral mucosa development. This study investigates Runx3 expression in oral tissues, revealing its presence in embryonic mouse tongue and palate epithelium.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Epithelial Biology

Background:

  • Mammalian oral mucosa development involves transforming growth factor beta (TGF-beta) and bone morphologic protein (BMP) signaling pathways.
  • Runx3, a runt-related transcription factor, is a known gastric tumor suppressor and regulator of epithelial cell growth and differentiation.
  • The Runx family, including Runx3, plays roles in mammalian development, with homologs like 'run' in C. elegans involved in gut and hypodermis development.

Purpose of the Study:

  • To examine the expression of Runx3, a downstream target of TGF-beta and BMP signaling, within the mammalian oral mucosa.
  • To investigate the role of Runx3 in the development of the tongue and palate epithelium.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) was employed to analyze Runx3 gene expression.

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  • Immunohistochemistry was utilized to detect Runx3 protein localization.
  • In situ hybridization was performed to determine the spatial expression pattern of Runx3.

    • Main Results:

    • Runx3 expression was detected in the oral mucosa of mouse embryos.
    • Specifically, Runx3 was found in the tongue and palate epithelium.
    • Expression was observed in mouse embryos from embryonic day 12.5 to 16.5.

    Conclusions:

    • Runx3 is expressed in the developing tongue and palate epithelium of mouse embryos.
    • The findings suggest a potential role for Runx3 in oral mucosa development.
    • Further investigation into the functional relationship between Runx3 and TGF-beta/BMP signaling in oral development is warranted.