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Updated: Aug 7, 2026

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis
Published on: March 28, 2025
[Protein-protein interaction between ESAT-6 and CFP-10 of mycobacterium tuberculosis]
Yan Li1, Lang Bao, Hui-dong Zhang
1Research Unit of Infection and Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the protein-protein interaction between ESAT-6 and CFP-10 of mycobacterium tuberculosis.
Methods:
ESAT-6 gene and CFP-10 gene were amplified from mycobacterium tuberculosis genome DNA. The ESAT-6 gene was subcloned into pGBKT7 and the CFP-10 gene was subcloned into pGADT7-Rec. After being verified with restriction endonuclease digestion and DNA sequencing, the recombinant vectors were transformed into yeast cell AH109 by lithium acetate method.
Results:
The yeast cells co-transformed with pGBKT7-ESAT-6 and pGADT7-CFP-10 grew on SD/-Ade/-His/-Leu/-Trp plates, and beta-galactosidase activity assays showed positive results.
Conclusion:
ESAT-6 and CFP-10 protein could interact with each other in yeast cells.
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