Related Experiment Video
Updated: Aug 7, 2026
Analysis and Imaging of Osteocytes
Published on: November 29, 2024
[Effect of fluid shear stress time on the morphological change of rat polarized osteoclasts]
Oing-hong Zhang1, Xing Liang, Qiang Dong
1Department of Prosthetics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To elucidate the morphological change of rat polarized osteoclasts influenced by different fluid shear stress time.
Methods:
Special TRAP dyeing and bone resorption of rat polarized osteoclasts were performed. A fluid shear stress device made in the authors' laboratory was used to enforce shear stress at different times on the polarized osteoclasts for observation on their morphological change and analysis on the kind of change by image analytical software. The time points were 0,15,30,45,60,75,90,105,120 min; the shear stress was 0.29 Pa.
Results:
Morphologically, the polarized osteoclasts were bigger than other monocytes. They were each about 30 microm in diameter, having about 20 round and black nuclei in cytoplasm. There were large numbers of cellular microfilaments. The areas and diameters of these osteoclasts increased with the times. Statistically, one-way ANOVA showed significant differences between the 0 min group and 30 min, 105 min groups respectively.
Conclusion:
In this study, the morphological change of polarized osteoclasts fluctuated with the increase of fluid shear stress time, and their areas and diameters exhibited a tendency to increase.
