Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Polyethylene glycol-mediated cell fusion.

Jian Yang¹, Ming Hong Shen

¹Institute for Stem Cell Research, University of Edinburgh, United Kingdom.

Methods in Molecular Biology (Clifton, N.J.)

|June 10, 2006

Summary

Polyethylene glycol (PEG)-mediated cell fusion offers an efficient method for creating somatic cell hybrids and enabling nuclear transfer. This protocol details PEG-mediated fusion techniques for various cell types and applications, including chromosome transfer.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Glassy dynamics and mechanical response in dense fluids of soft repulsive spheres. I. Activated relaxation, kinetic vitrification, and fragility.

The Journal of chemical physics·2011

Same author

Climatic reconstruction at the Miocene Shanwang basin, China, using leaf margin analysis, CLAMP, coexistence approach, and overlapping distribution analysis.

American journal of botany·2011

Same author

Novel candidate colorectal cancer biomarkers identified by methylation microarray-based scanning.

Endocrine-related cancer·2011

Same author

Stress and strain analysis of contractions during ramp distension in partially obstructed guinea pig jejunal segments.

Journal of biomechanics·2011

Same author

Role of Gα(12)- and Gα(13)-protein subunit linkage of D(3) dopamine receptors in the natriuretic effect of D(3) dopamine receptor in kidney.

Hypertension research : official journal of the Japanese Society of Hypertension·2011

Same author

Transarticular screw and C1 hook fixation for os odontoideum with atlantoaxial dislocation.

World neurosurgery·2011

Area of Science:

Cell Biology
Molecular Biology
Biotechnology

Background:

Polyethylene glycol (PEG)-mediated cell fusion is a widely used, efficient technique.
It is crucial for producing somatic cell hybrids and nuclear transfer in mammalian cloning.

Purpose of the Study:

To describe a basic protocol for PEG-mediated cell fusion for somatic cell hybrid production.
To provide guidelines for adapting PEG-mediated cell fusion technology for broader research applications.

Main Methods:

Describes PEG-mediated fusion protocols adaptable for adherent and suspension cells.
Utilizes whole cells or microcells as donor cells for fusion with recipient cells.
Highlights microcell fusion for targeted chromosome transfer between diverse cell types.

Main Results:

Successfully introduced mammalian minichromosomes into various vertebrate cells using microcell fusion.
Demonstrated the versatility of PEG-mediated cell fusion for different cell combinations.

Conclusions:

The described protocol provides a foundational guideline for PEG-mediated cell fusion.
This technique is adaptable for various cell fusion research, including chromosome transfer and hybrid production.

Related Experiment Videos