Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analyzing histone modification using crosslinked chromatin treated with micrococcal nuclease.

Pascal Lefevre1, Constanze Bonifer

  • 1University of Leeds, St. James's University Hospital, Molecular Unit, Leeds, United Kingdom.

Methods in Molecular Biology (Clifton, N.J.)
|June 10, 2006
PubMed
Summary

Researchers developed a new chromatin immunoprecipitation method to study localized histone modifications. This technique helps understand gene expression patterns by isolating small chromatin fragments and minimizing histone movement during preparation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanistic insights into gene regulation driving normal and malignant hematopoietic development from embryonic and induced pluripotent stem cell differentiation.

Experimental hematology·2026
Same author

Fusion gene depletion eliminates stemness and induces bidirectional differentiation of acute myeloid leukemia.

Blood·2025
Same author

A heterozygous CEBPA mutation disrupting the bZIP domain in a RUNX1 and SRSF2 mutational background causes MDS disease progression.

Nature communications·2025
Same author

Fusion-specific chromatin profiles mediate transcriptional heterogeneity in MLL-rearranged acute lymphoblastic leukemia.

Blood advances·2024
Same author

Regulation of developmentally controlled enhancer activity by extrinsic signals in normal and malignant cells: AP-1 at the centre.

Frontiers in epigenetics and epigenomics·2024
Same author

Gene regulatory network and signalling pathway rewiring: How blood cancer cells shift their shapes to evade drug treatment.

Oncotarget·2024

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Epigenetic processes regulate gene expression through histone modifications.
  • Histone modifications influence chromatin structure and serve as binding sites for regulatory proteins.
  • Understanding these modifications is key to deciphering gene expression patterns.

Purpose of the Study:

  • To develop a chromatin immunoprecipitation (ChIP) procedure for analyzing localized histone modifications.
  • To enable the study of histone modifications at specific genomic regions, even when involving few nucleosomes.

Main Methods:

  • Established a chromatin immunoprecipitation procedure.
  • Utilized crosslinked chromatin for improved sample preparation.
  • Focused on isolating small chromatin fragments.

Related Experiment Videos

Main Results:

  • The developed ChIP procedure allows for the isolation of small chromatin fragments.
  • The method minimizes histone movement during chromatin preparation.
  • This facilitates the examination of localized histone modification patterns.

Conclusions:

  • The new ChIP technique provides a robust method for studying localized histone modifications.
  • This advancement aids in understanding the role of histone modifications in establishing gene expression patterns.