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[Effect of advanced oxidation protein products on nitric oxide production in mouse peritoneal macrophages]
Zhong-hai Li1, Shang-xi Liu, Fan-fan Hou
1Department of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. loy5460@yahoo.com.
Objective:
To investigate the effect of advanced oxidation protein products (AOPP) on nitric oxide (NO) production in mouse peritoneal macrophages (MPMs).
Methods:
MPMs were incubated in the absence or presence of lipopolysaccharide (LPS) with AOPP-modified bovine serum albumin (BSA) prepared by exposure of BSA to hypoclorous acid or pre-treated with AOPP-BSA and subsequent stimulation with LPS. NO production in the supernatants of the culture media was determined spectrophotometrically using Griess method. The cell viability was measured by MTT assay.
Results:
BSA induced significant NO production in MPMs. AOPP modification of BSA significantly inhibited NO production, and AOPP-BSA exhibited time- and dose-dependent inhibition of NO production induced by LPS in MPMs incubated together with LPS or pre-treated before LPS stimulation.
Conclusion:
AOPP-BSA is capable of inhibiting inducible NO production in MPMs.
Insights
Advanced oxidation protein products (AOPP) significantly inhibit nitric oxide (NO) production in mouse peritoneal macrophages (MPMs). This inhibitory effect of AOPP-modified bovine serum albumin (AOPP-BSA) is dose- and time-dependent.
Area of Science:
- Immunology
- Biochemistry
- Cell Biology
Background:
- Nitric oxide (NO) plays a crucial role in immune responses and inflammation.
- Advanced oxidation protein products (AOPP) are biomarkers of oxidative stress implicated in various diseases.
- The impact of AOPP on NO production in macrophages requires further elucidation.
Purpose of the Study:
- To investigate the effect of AOPP on nitric oxide (NO) production in mouse peritoneal macrophages (MPMs).
- To determine if AOPP-BSA can modulate LPS-induced NO production in MPMs.
Main Methods:
- Mouse peritoneal macrophages (MPMs) were cultured with or without lipopolysaccharide (LPS).
- MPMs were treated with AOPP-modified bovine serum albumin (AOPP-BSA) alone or in combination with LPS.
- Nitric oxide (NO) production was quantified using the Griess assay, and cell viability was assessed via MTT assay.
Main Results:
- Bovine serum albumin (BSA) alone induced significant NO production in MPMs.
- AOPP modification of BSA markedly inhibited NO production.
- AOPP-BSA demonstrated a time- and dose-dependent inhibition of LPS-induced NO production in MPMs.
Conclusions:
- AOPP-modified bovine serum albumin (AOPP-BSA) effectively inhibits inducible nitric oxide production in mouse peritoneal macrophages (MPMs).
- These findings suggest a potential role for AOPP in modulating inflammatory responses mediated by NO.
